Search results for "soft X-ray"
showing 10 items of 17 documents
Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo
2016
Summary - The realization that nuclear distribution of DNA, RNA, and proteins differs between cell types and developmental stages suggests that nuclear organization serves regulatory functions. Understanding the logic of nuclear architecture and how it contributes to differentiation and cell fate commitment remains challenging. Here, we use soft X-ray tomography (SXT) to image chromatin organization, distribution, and biophysical properties during neurogenesis in vivo. Our analyses reveal that chromatin with similar biophysical properties forms an elaborate connected network throughout the entire nucleus. Although this interconnectivity is present in every developmental stage, differentiati…
Compact Cell Imaging Device (CoCID) provides insights into the cellular origins of viral infections
2021
The overall CoCID concept is centred on providing virologists with a next-generation imaging device, which, through increased penetration and depth of focus, as well as through high natural contrast and sensitivity to organelle density (including virus-related organelles), will produce higher-fidelity ultrastructural images of whole intact cells. These insights will, in turn, help increase our understanding of the links between the structural reorganisation of cells and the mechanisms of viral entry, replication, assembly, and egress in cells. CoCID will provide this valuable imaging capability in the form of a compact lab-scale device that will greatly improve the accessibility of soft X-r…
Putting molecules in their place.
2014
Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X‐ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high‐resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near‐native, cryopreserved state. Here we…
Visible light apparatus for preliminary tests of x-ray optics
2006
We report a useful visible light testing procedure for a first analysis of soft X-ray grazing incidence optics (0.1-2 keV). Although diffraction is a limit in the application of this method, great advantages are obtained by running the tests in air with direct access to modify the geometrical mounting of the individual mirror shells. We present the experimental apparatus and show the first results of the investigation of light weight optics based on plastic foil material and comparison with results obtained with an X-ray beam.
High-Resolution Spectroscopy in Fast Atomic Beams
1977
Narrow optical resonances have been observed in fast beams of Na and Cs atoms, obtained from ion beams by charge-transfer collisions with Na, K, or Cs. Corresponding to the narrowing of the velocity distribution, occurring by acceleration, the Doppler width along the beam direction is considerably reduced [1, 2].
Soft X-ray emission spectroscopy used for the characterization of a-C and CNx thin films
2015
Abstract We present the results of a soft X-ray emission spectroscopy study of a-C and CNx films on a Si(100) substrate. Also for the characterization of the homogeneity in depth of these films electron energy loss spectroscopy measurements with localization better than 4 nm were carried out. In case of CNx films the highest diamond-like modification occurs in the region close to the Si(100) substrate. The film density decreases with increasing distance from the substrate and becomes almost constant in range of thicknesses more than ~ 2 nm.
Second order reflection from crystals used in soft X-ray spectroscopy
2015
Abstract In this note the ratio of the second to the first order reflection is determined for the KAP and PbSt crystals, for wavelengths corresponding to the Al K-line emission. The source of the radiation was a low-voltage stabilized X-ray tube. The X-rays were detected with a Bragg spectrometer equipped with a proportional counter detector. The signal measured by the proportional counter was subsequently pulse height analyzed.
4D texture of circular dichroism in soft-x-ray photoemission from tungsten
2019
Brief treatment and crisis intervention 21(1), 013017 (2019). doi:10.1088/1367-2630/aaf4cd
Study and implementation of a soft X-ray 100 eV -20 keV fixed exit monochromator system
2006
We describe a “built in house” X-ray monochromator which produces a fixed exit X-ray beam tunable in the full energy range 0.1 - 20 keV. The system is based on a double diffraction on two large size parallel crystals positioned using a remotely controlled micropositioning system in order to keep the position of the monochromatic beam for any chosen energy. Up to six different diffracting elements can be selected without breaking the vacuum. This allows to cover the full energy range of interest. The system is part of an upgrading project of the XACT facility at the Istituto Nazionale di Astrofisica - Osservatorio Astronomico di Palermo G.S. Vaiana, and will be employed for the testing and c…
Optical Spectroscopy of Short-Lived Isotopes
1979
This chapter will concentrate on a special branch of the large field of optical spectroscopy, namely, the study of hyperfine structure (hfs) and isotope shift (IS) of short-lived isotopes. Such investigations give information on some basic properties of these nuclei: the spin ( ), the magnetic moment (μI), the spectroscopic quadrupole moment (Q s ), and the change of the mean square charge radius between different isotopes (δ〈r 2〉).